Sergey Krylov

Sergey Krylov headshotPhD (Moscow State University)
Canada Research Chair and Professor
E–mail: skrylov@yorku.ca
Website: www.chem.yorku.ca/profs/krylov/
Research areas: Biomolecular interactions, Personalized cancer medicine, High-throughput drug screening, Continuous-flow chemistry, Ultra-sensitive chemical analysis

Research Focus

The research in Krylov's laboratory is conducted by an interdisciplinary group of scientists with a broad range of interests, spanning physical and life sciences. We foster this synergy of expertise to achieve comprehensive interrogation of multifaceted scientific problems. The overarching goal of our research is to create innovative and enabling technologies for biomedical and bioanalytical applications. This process includes the study of basic natural phenomena, development and validation of new methods, and creation of practical tools for use in clinical and industrial settings. The experimental work in our lab is performed on all levels of complexity, from in vitro and in silica studies, to tissue and cell-culture work, and in vivo animal models. Furthermore, our long-standing collaborations with multiple research hospitals provide us with access to human clinical samples. The team operates a state-of-the-art infrastructure, and has attained proficiency in a wide range of advanced techniques, including analytical separation methods (Capillary Electrophoresis and HPLC), Mass Spectrometry, High-Content Imaging, Confocal Microscopy, Flow and Chemical Cytometry, and Whole-Body Imaging. The laboratory hosts a prototype manufacturing facility, which allows us to implement our ideas into novel instrumentation. Lastly, the lab is a part of the Centre for Research on Biomolecular Interactions, with their facilities available for our use.

Representative publications:

Krylov, S.N.; Arriaga, E.; Zhang, Z.; Dovichi, N.J.Instrumentation for chemical cytometry. Anal. Chem. 2000, 72, 872-877.

Krylov, S.N.; Dovichi N.J. Capillary electrophoresis for the analysis of biopolymers (fundamental review). Anal. Chem. 2000, 72, 111R-128R.

Krylov, S.N.; Zhang, Z.; Chan, N.W.C.; Arriaga, E.; Palcic, M.M.; Dovichi, N.J. Correlating cell cycle with metabolism in single cells: combination of image and metabolic cytometry. Cytometry 1999, 37, 14-20.

Zhang, Z.; Krylov, S.N.; Arriaga, E.; Dovichi, N.J. One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell. Anal. Chem. 2000, 72, 318-322.